Application

Immunocytochemistry Analysis: A representative lot detected Perilipin-3, C-terminus in OP9 cells (Skinner, J. R., et al. (2009). J Biol Chem. 284(45):30941-30948).

This Anti-Perilipin-3 antibody, C-terminus is validated for use in western blotting & ICC for the detection of Perilipin-3.

Research CategorySignaling

Research Sub CategorySignaling Neuroscience

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Perilipin-3, also known as Cargo selection protein TIP47, or Placental protein 17, PP17, 47 kDa mannose 6-phosphate receptor-binding protein, 47 kDa MPR-binding protein, also known as Mannose-6-phosphate receptor-binding protein 1, and encoded by the gene PLIN3/M6prbp1/Tip47, is a peripheral membrane protein that is required for the transport of mannose 6-phosphate receptors (MPR) from the endosomes to the trans-Golgi network. Perilipin 3 is part of the Perilipin family of proteins which are proteins that decorate the surface of cytosolic lipid droplets and play important roles in lipid metabolism. Cytosolic lipid droplets are now recognized as multifunctional organelles that, among other things, can accumulate in leukocytes under various inflammatory conditions. Perilipin-3 seems to play a critical role in the formation of lipid droplets and production of the the prostaglandin PGE2 an important inflammatory compound.

Immunogen

Linear peptide corresponding to the C-terminus of mouse Perilipin-3, C-terminus.

Epitope: C-terminus

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Affinity purified

Purified rabbit polyclonal in buffer containing PBS without preservatives with 10% glycerol.

Quality

Evaluated by Western Blotting in NIH/3T3-L1 cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Perilipin-3, C-terminus in 10 µg of NIH/3T3-L1 cell lysate.

Storage and Stability

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

Target description

~ 52 kDa observed